Honduran military police opened fire on students protesting at a university in the capital, Tegucigalpa, injuring at least four people.
Hundreds of people, many wearing face masks, were demanding the resignation of President Juan Orlando Hernández amid growing anti-government protests. The government deployed the army and military police across the country last week after clashes left three dead.
The demonstrations were sparked by proposed health and education reforms.
The conservative president, who enjoys the staunch support of the United States administration, is also accused of becoming increasingly authoritarian. He changed the constitution to stand for a second four-year term and was re-elected in 2017, in a vote heavily criticised by opponents and international observers.
Thousands of people have fled Honduras as well as its neighbours El Salvador and Guatemala to the US in recent years because of violence and economic instability. Some 40 military police entered the campus of the National Autonomous University of Honduras after firing tear gas on protesters, who responded by throwing stones, AFP news agency reports.
Riot police then chased the demonstrators and opened fire with rifles. All the injured were out of danger, according to a hospital spokeswoman.